digital electronic weighing scale seca 780 Search Results


electronic column scale seca-780  (Seca)
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Seca electronic column scale seca-780
Electronic Column Scale Seca 780, supplied by Seca, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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portable scale seca 780/783  (Seca)
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Portable Scale Seca 780/783, supplied by Seca, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scanning probe confocal microscope zeiss lsm 780  (Carl Zeiss)
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Carl Zeiss scanning probe confocal microscope zeiss lsm 780
(A) Representative phase contrast images showing morphology of MUC1 FACS sorted MCF7 cells cultured on E-selectin coated substrate. MCF7 cells are sorted in mucin-1 negative, intermediate (low) and high MUC1 expressing cell populations and were grown for 14 hours in presence of DMSO control or 0.4 U/ml Neuraminidase and 20 µg/ml tunicamycin (NMase). (B) Quantification of cell spread area and cell circularity form obtained images (n>120 cells from 3 independent experiments, ns p > 0.05, ** p < 0.005, data is presented as mean ± SEM). (C) Representative trajectories of FACS sorted MCF7 cells migrating on E-selectin coated cell culture plate in the presence of 0.4 U/ml neuraminidase and 20 µg/ml Tunicamycin (NMase) or vehicle (DMSO). (D) Quantification of 2D speed (n=3, 55-70 cells per condition, ** p < 0.005, data is presented as mean ± SEM). (E) Adhesion assay with MUC1 FACS sorted MCF7 cells on E-selectin coated surface. Cells seeded on coated 96-well plate were allowed to attach for 20 minutes, was then PBS washed and was stained with DAPI and the whole plate was imaged using <t>microscope</t> to count cells. Representative images of nucleus in one well after thresholding with top row showing wells without wash step (seed) and the bottom panel showing attached cells with and without NMase treatment. (F) Quantification of attached cell . (n=3, ** p < 0.005, ns p > 0.05, data is presented as mean ± SEM). (G) FACS sorted MCF7 cells seeded on a selectin coated microfluidics channel were subjected to different flow induced shear stress and were imaged using a live cell imaging setup. Representative phase contrast images showing a section of the channel at different time points with attached cells (H) Quantification of remaining attached cell fraction across different conditions as they are subjected to flow induced shear stress (n≥3 data is presented as mean ± SEM). (I) WGA staining shows cell leave cell leaves glycan patch after detachment. WGA stained MCF7 cells seeded on a selectin coated microfluidics channel subjected flow induced shear was imaged using fluorescent live cell imaging setup. Yellow dotted line shows the cell boundary and orange dotted line shows initial cell adhesion area.
Scanning Probe Confocal Microscope Zeiss Lsm 780, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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780 electronic balance scale  (Seca)
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Seca 780 electronic balance scale
(A) Representative phase contrast images showing morphology of MUC1 FACS sorted MCF7 cells cultured on E-selectin coated substrate. MCF7 cells are sorted in mucin-1 negative, intermediate (low) and high MUC1 expressing cell populations and were grown for 14 hours in presence of DMSO control or 0.4 U/ml Neuraminidase and 20 µg/ml tunicamycin (NMase). (B) Quantification of cell spread area and cell circularity form obtained images (n>120 cells from 3 independent experiments, ns p > 0.05, ** p < 0.005, data is presented as mean ± SEM). (C) Representative trajectories of FACS sorted MCF7 cells migrating on E-selectin coated cell culture plate in the presence of 0.4 U/ml neuraminidase and 20 µg/ml Tunicamycin (NMase) or vehicle (DMSO). (D) Quantification of 2D speed (n=3, 55-70 cells per condition, ** p < 0.005, data is presented as mean ± SEM). (E) Adhesion assay with MUC1 FACS sorted MCF7 cells on E-selectin coated surface. Cells seeded on coated 96-well plate were allowed to attach for 20 minutes, was then PBS washed and was stained with DAPI and the whole plate was imaged using <t>microscope</t> to count cells. Representative images of nucleus in one well after thresholding with top row showing wells without wash step (seed) and the bottom panel showing attached cells with and without NMase treatment. (F) Quantification of attached cell . (n=3, ** p < 0.005, ns p > 0.05, data is presented as mean ± SEM). (G) FACS sorted MCF7 cells seeded on a selectin coated microfluidics channel were subjected to different flow induced shear stress and were imaged using a live cell imaging setup. Representative phase contrast images showing a section of the channel at different time points with attached cells (H) Quantification of remaining attached cell fraction across different conditions as they are subjected to flow induced shear stress (n≥3 data is presented as mean ± SEM). (I) WGA staining shows cell leave cell leaves glycan patch after detachment. WGA stained MCF7 cells seeded on a selectin coated microfluidics channel subjected flow induced shear was imaged using fluorescent live cell imaging setup. Yellow dotted line shows the cell boundary and orange dotted line shows initial cell adhesion area.
780 Electronic Balance Scale, supplied by Seca, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nude body mass seca 780  (Seca)
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(A) Representative phase contrast images showing morphology of MUC1 FACS sorted MCF7 cells cultured on E-selectin coated substrate. MCF7 cells are sorted in mucin-1 negative, intermediate (low) and high MUC1 expressing cell populations and were grown for 14 hours in presence of DMSO control or 0.4 U/ml Neuraminidase and 20 µg/ml tunicamycin (NMase). (B) Quantification of cell spread area and cell circularity form obtained images (n>120 cells from 3 independent experiments, ns p > 0.05, ** p < 0.005, data is presented as mean ± SEM). (C) Representative trajectories of FACS sorted MCF7 cells migrating on E-selectin coated cell culture plate in the presence of 0.4 U/ml neuraminidase and 20 µg/ml Tunicamycin (NMase) or vehicle (DMSO). (D) Quantification of 2D speed (n=3, 55-70 cells per condition, ** p < 0.005, data is presented as mean ± SEM). (E) Adhesion assay with MUC1 FACS sorted MCF7 cells on E-selectin coated surface. Cells seeded on coated 96-well plate were allowed to attach for 20 minutes, was then PBS washed and was stained with DAPI and the whole plate was imaged using <t>microscope</t> to count cells. Representative images of nucleus in one well after thresholding with top row showing wells without wash step (seed) and the bottom panel showing attached cells with and without NMase treatment. (F) Quantification of attached cell . (n=3, ** p < 0.005, ns p > 0.05, data is presented as mean ± SEM). (G) FACS sorted MCF7 cells seeded on a selectin coated microfluidics channel were subjected to different flow induced shear stress and were imaged using a live cell imaging setup. Representative phase contrast images showing a section of the channel at different time points with attached cells (H) Quantification of remaining attached cell fraction across different conditions as they are subjected to flow induced shear stress (n≥3 data is presented as mean ± SEM). (I) WGA staining shows cell leave cell leaves glycan patch after detachment. WGA stained MCF7 cells seeded on a selectin coated microfluidics channel subjected flow induced shear was imaged using fluorescent live cell imaging setup. Yellow dotted line shows the cell boundary and orange dotted line shows initial cell adhesion area.
Nude Body Mass Seca 780, supplied by Seca, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scale height  (Seca)
86
Seca scale height
(A) Representative phase contrast images showing morphology of MUC1 FACS sorted MCF7 cells cultured on E-selectin coated substrate. MCF7 cells are sorted in mucin-1 negative, intermediate (low) and high MUC1 expressing cell populations and were grown for 14 hours in presence of DMSO control or 0.4 U/ml Neuraminidase and 20 µg/ml tunicamycin (NMase). (B) Quantification of cell spread area and cell circularity form obtained images (n>120 cells from 3 independent experiments, ns p > 0.05, ** p < 0.005, data is presented as mean ± SEM). (C) Representative trajectories of FACS sorted MCF7 cells migrating on E-selectin coated cell culture plate in the presence of 0.4 U/ml neuraminidase and 20 µg/ml Tunicamycin (NMase) or vehicle (DMSO). (D) Quantification of 2D speed (n=3, 55-70 cells per condition, ** p < 0.005, data is presented as mean ± SEM). (E) Adhesion assay with MUC1 FACS sorted MCF7 cells on E-selectin coated surface. Cells seeded on coated 96-well plate were allowed to attach for 20 minutes, was then PBS washed and was stained with DAPI and the whole plate was imaged using <t>microscope</t> to count cells. Representative images of nucleus in one well after thresholding with top row showing wells without wash step (seed) and the bottom panel showing attached cells with and without NMase treatment. (F) Quantification of attached cell . (n=3, ** p < 0.005, ns p > 0.05, data is presented as mean ± SEM). (G) FACS sorted MCF7 cells seeded on a selectin coated microfluidics channel were subjected to different flow induced shear stress and were imaged using a live cell imaging setup. Representative phase contrast images showing a section of the channel at different time points with attached cells (H) Quantification of remaining attached cell fraction across different conditions as they are subjected to flow induced shear stress (n≥3 data is presented as mean ± SEM). (I) WGA staining shows cell leave cell leaves glycan patch after detachment. WGA stained MCF7 cells seeded on a selectin coated microfluidics channel subjected flow induced shear was imaged using fluorescent live cell imaging setup. Yellow dotted line shows the cell boundary and orange dotted line shows initial cell adhesion area.
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portable digital scale model 780  (Seca)
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Seca portable digital scale model 780
(A) Representative phase contrast images showing morphology of MUC1 FACS sorted MCF7 cells cultured on E-selectin coated substrate. MCF7 cells are sorted in mucin-1 negative, intermediate (low) and high MUC1 expressing cell populations and were grown for 14 hours in presence of DMSO control or 0.4 U/ml Neuraminidase and 20 µg/ml tunicamycin (NMase). (B) Quantification of cell spread area and cell circularity form obtained images (n>120 cells from 3 independent experiments, ns p > 0.05, ** p < 0.005, data is presented as mean ± SEM). (C) Representative trajectories of FACS sorted MCF7 cells migrating on E-selectin coated cell culture plate in the presence of 0.4 U/ml neuraminidase and 20 µg/ml Tunicamycin (NMase) or vehicle (DMSO). (D) Quantification of 2D speed (n=3, 55-70 cells per condition, ** p < 0.005, data is presented as mean ± SEM). (E) Adhesion assay with MUC1 FACS sorted MCF7 cells on E-selectin coated surface. Cells seeded on coated 96-well plate were allowed to attach for 20 minutes, was then PBS washed and was stained with DAPI and the whole plate was imaged using <t>microscope</t> to count cells. Representative images of nucleus in one well after thresholding with top row showing wells without wash step (seed) and the bottom panel showing attached cells with and without NMase treatment. (F) Quantification of attached cell . (n=3, ** p < 0.005, ns p > 0.05, data is presented as mean ± SEM). (G) FACS sorted MCF7 cells seeded on a selectin coated microfluidics channel were subjected to different flow induced shear stress and were imaged using a live cell imaging setup. Representative phase contrast images showing a section of the channel at different time points with attached cells (H) Quantification of remaining attached cell fraction across different conditions as they are subjected to flow induced shear stress (n≥3 data is presented as mean ± SEM). (I) WGA staining shows cell leave cell leaves glycan patch after detachment. WGA stained MCF7 cells seeded on a selectin coated microfluidics channel subjected flow induced shear was imaged using fluorescent live cell imaging setup. Yellow dotted line shows the cell boundary and orange dotted line shows initial cell adhesion area.
Portable Digital Scale Model 780, supplied by Seca, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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780 digital scale  (Seca)
90
Seca 780 digital scale
(A) Representative phase contrast images showing morphology of MUC1 FACS sorted MCF7 cells cultured on E-selectin coated substrate. MCF7 cells are sorted in mucin-1 negative, intermediate (low) and high MUC1 expressing cell populations and were grown for 14 hours in presence of DMSO control or 0.4 U/ml Neuraminidase and 20 µg/ml tunicamycin (NMase). (B) Quantification of cell spread area and cell circularity form obtained images (n>120 cells from 3 independent experiments, ns p > 0.05, ** p < 0.005, data is presented as mean ± SEM). (C) Representative trajectories of FACS sorted MCF7 cells migrating on E-selectin coated cell culture plate in the presence of 0.4 U/ml neuraminidase and 20 µg/ml Tunicamycin (NMase) or vehicle (DMSO). (D) Quantification of 2D speed (n=3, 55-70 cells per condition, ** p < 0.005, data is presented as mean ± SEM). (E) Adhesion assay with MUC1 FACS sorted MCF7 cells on E-selectin coated surface. Cells seeded on coated 96-well plate were allowed to attach for 20 minutes, was then PBS washed and was stained with DAPI and the whole plate was imaged using <t>microscope</t> to count cells. Representative images of nucleus in one well after thresholding with top row showing wells without wash step (seed) and the bottom panel showing attached cells with and without NMase treatment. (F) Quantification of attached cell . (n=3, ** p < 0.005, ns p > 0.05, data is presented as mean ± SEM). (G) FACS sorted MCF7 cells seeded on a selectin coated microfluidics channel were subjected to different flow induced shear stress and were imaged using a live cell imaging setup. Representative phase contrast images showing a section of the channel at different time points with attached cells (H) Quantification of remaining attached cell fraction across different conditions as they are subjected to flow induced shear stress (n≥3 data is presented as mean ± SEM). (I) WGA staining shows cell leave cell leaves glycan patch after detachment. WGA stained MCF7 cells seeded on a selectin coated microfluidics channel subjected flow induced shear was imaged using fluorescent live cell imaging setup. Yellow dotted line shows the cell boundary and orange dotted line shows initial cell adhesion area.
780 Digital Scale, supplied by Seca, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scale  (Seca)
86
Seca scale
(A) Representative phase contrast images showing morphology of MUC1 FACS sorted MCF7 cells cultured on E-selectin coated substrate. MCF7 cells are sorted in mucin-1 negative, intermediate (low) and high MUC1 expressing cell populations and were grown for 14 hours in presence of DMSO control or 0.4 U/ml Neuraminidase and 20 µg/ml tunicamycin (NMase). (B) Quantification of cell spread area and cell circularity form obtained images (n>120 cells from 3 independent experiments, ns p > 0.05, ** p < 0.005, data is presented as mean ± SEM). (C) Representative trajectories of FACS sorted MCF7 cells migrating on E-selectin coated cell culture plate in the presence of 0.4 U/ml neuraminidase and 20 µg/ml Tunicamycin (NMase) or vehicle (DMSO). (D) Quantification of 2D speed (n=3, 55-70 cells per condition, ** p < 0.005, data is presented as mean ± SEM). (E) Adhesion assay with MUC1 FACS sorted MCF7 cells on E-selectin coated surface. Cells seeded on coated 96-well plate were allowed to attach for 20 minutes, was then PBS washed and was stained with DAPI and the whole plate was imaged using <t>microscope</t> to count cells. Representative images of nucleus in one well after thresholding with top row showing wells without wash step (seed) and the bottom panel showing attached cells with and without NMase treatment. (F) Quantification of attached cell . (n=3, ** p < 0.005, ns p > 0.05, data is presented as mean ± SEM). (G) FACS sorted MCF7 cells seeded on a selectin coated microfluidics channel were subjected to different flow induced shear stress and were imaged using a live cell imaging setup. Representative phase contrast images showing a section of the channel at different time points with attached cells (H) Quantification of remaining attached cell fraction across different conditions as they are subjected to flow induced shear stress (n≥3 data is presented as mean ± SEM). (I) WGA staining shows cell leave cell leaves glycan patch after detachment. WGA stained MCF7 cells seeded on a selectin coated microfluidics channel subjected flow induced shear was imaged using fluorescent live cell imaging setup. Yellow dotted line shows the cell boundary and orange dotted line shows initial cell adhesion area.
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model 780 seca digital scale balance  (Seca)
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(A) Representative phase contrast images showing morphology of MUC1 FACS sorted MCF7 cells cultured on E-selectin coated substrate. MCF7 cells are sorted in mucin-1 negative, intermediate (low) and high MUC1 expressing cell populations and were grown for 14 hours in presence of DMSO control or 0.4 U/ml Neuraminidase and 20 µg/ml tunicamycin (NMase). (B) Quantification of cell spread area and cell circularity form obtained images (n>120 cells from 3 independent experiments, ns p > 0.05, ** p < 0.005, data is presented as mean ± SEM). (C) Representative trajectories of FACS sorted MCF7 cells migrating on E-selectin coated cell culture plate in the presence of 0.4 U/ml neuraminidase and 20 µg/ml Tunicamycin (NMase) or vehicle (DMSO). (D) Quantification of 2D speed (n=3, 55-70 cells per condition, ** p < 0.005, data is presented as mean ± SEM). (E) Adhesion assay with MUC1 FACS sorted MCF7 cells on E-selectin coated surface. Cells seeded on coated 96-well plate were allowed to attach for 20 minutes, was then PBS washed and was stained with DAPI and the whole plate was imaged using <t>microscope</t> to count cells. Representative images of nucleus in one well after thresholding with top row showing wells without wash step (seed) and the bottom panel showing attached cells with and without NMase treatment. (F) Quantification of attached cell . (n=3, ** p < 0.005, ns p > 0.05, data is presented as mean ± SEM). (G) FACS sorted MCF7 cells seeded on a selectin coated microfluidics channel were subjected to different flow induced shear stress and were imaged using a live cell imaging setup. Representative phase contrast images showing a section of the channel at different time points with attached cells (H) Quantification of remaining attached cell fraction across different conditions as they are subjected to flow induced shear stress (n≥3 data is presented as mean ± SEM). (I) WGA staining shows cell leave cell leaves glycan patch after detachment. WGA stained MCF7 cells seeded on a selectin coated microfluidics channel subjected flow induced shear was imaged using fluorescent live cell imaging setup. Yellow dotted line shows the cell boundary and orange dotted line shows initial cell adhesion area.
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serial fluorescence images  (Omega Optical)
90
Omega Optical serial fluorescence images
(A) Representative phase contrast images showing morphology of MUC1 FACS sorted MCF7 cells cultured on E-selectin coated substrate. MCF7 cells are sorted in mucin-1 negative, intermediate (low) and high MUC1 expressing cell populations and were grown for 14 hours in presence of DMSO control or 0.4 U/ml Neuraminidase and 20 µg/ml tunicamycin (NMase). (B) Quantification of cell spread area and cell circularity form obtained images (n>120 cells from 3 independent experiments, ns p > 0.05, ** p < 0.005, data is presented as mean ± SEM). (C) Representative trajectories of FACS sorted MCF7 cells migrating on E-selectin coated cell culture plate in the presence of 0.4 U/ml neuraminidase and 20 µg/ml Tunicamycin (NMase) or vehicle (DMSO). (D) Quantification of 2D speed (n=3, 55-70 cells per condition, ** p < 0.005, data is presented as mean ± SEM). (E) Adhesion assay with MUC1 FACS sorted MCF7 cells on E-selectin coated surface. Cells seeded on coated 96-well plate were allowed to attach for 20 minutes, was then PBS washed and was stained with DAPI and the whole plate was imaged using <t>microscope</t> to count cells. Representative images of nucleus in one well after thresholding with top row showing wells without wash step (seed) and the bottom panel showing attached cells with and without NMase treatment. (F) Quantification of attached cell . (n=3, ** p < 0.005, ns p > 0.05, data is presented as mean ± SEM). (G) FACS sorted MCF7 cells seeded on a selectin coated microfluidics channel were subjected to different flow induced shear stress and were imaged using a live cell imaging setup. Representative phase contrast images showing a section of the channel at different time points with attached cells (H) Quantification of remaining attached cell fraction across different conditions as they are subjected to flow induced shear stress (n≥3 data is presented as mean ± SEM). (I) WGA staining shows cell leave cell leaves glycan patch after detachment. WGA stained MCF7 cells seeded on a selectin coated microfluidics channel subjected flow induced shear was imaged using fluorescent live cell imaging setup. Yellow dotted line shows the cell boundary and orange dotted line shows initial cell adhesion area.
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univ paris sud, inserm unit 780, cardiovasc epidemiol sect  (Inserm Transfert)
90
Inserm Transfert univ paris sud, inserm unit 780, cardiovasc epidemiol sect
(A) Representative phase contrast images showing morphology of MUC1 FACS sorted MCF7 cells cultured on E-selectin coated substrate. MCF7 cells are sorted in mucin-1 negative, intermediate (low) and high MUC1 expressing cell populations and were grown for 14 hours in presence of DMSO control or 0.4 U/ml Neuraminidase and 20 µg/ml tunicamycin (NMase). (B) Quantification of cell spread area and cell circularity form obtained images (n>120 cells from 3 independent experiments, ns p > 0.05, ** p < 0.005, data is presented as mean ± SEM). (C) Representative trajectories of FACS sorted MCF7 cells migrating on E-selectin coated cell culture plate in the presence of 0.4 U/ml neuraminidase and 20 µg/ml Tunicamycin (NMase) or vehicle (DMSO). (D) Quantification of 2D speed (n=3, 55-70 cells per condition, ** p < 0.005, data is presented as mean ± SEM). (E) Adhesion assay with MUC1 FACS sorted MCF7 cells on E-selectin coated surface. Cells seeded on coated 96-well plate were allowed to attach for 20 minutes, was then PBS washed and was stained with DAPI and the whole plate was imaged using <t>microscope</t> to count cells. Representative images of nucleus in one well after thresholding with top row showing wells without wash step (seed) and the bottom panel showing attached cells with and without NMase treatment. (F) Quantification of attached cell . (n=3, ** p < 0.005, ns p > 0.05, data is presented as mean ± SEM). (G) FACS sorted MCF7 cells seeded on a selectin coated microfluidics channel were subjected to different flow induced shear stress and were imaged using a live cell imaging setup. Representative phase contrast images showing a section of the channel at different time points with attached cells (H) Quantification of remaining attached cell fraction across different conditions as they are subjected to flow induced shear stress (n≥3 data is presented as mean ± SEM). (I) WGA staining shows cell leave cell leaves glycan patch after detachment. WGA stained MCF7 cells seeded on a selectin coated microfluidics channel subjected flow induced shear was imaged using fluorescent live cell imaging setup. Yellow dotted line shows the cell boundary and orange dotted line shows initial cell adhesion area.
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Image Search Results


(A) Representative phase contrast images showing morphology of MUC1 FACS sorted MCF7 cells cultured on E-selectin coated substrate. MCF7 cells are sorted in mucin-1 negative, intermediate (low) and high MUC1 expressing cell populations and were grown for 14 hours in presence of DMSO control or 0.4 U/ml Neuraminidase and 20 µg/ml tunicamycin (NMase). (B) Quantification of cell spread area and cell circularity form obtained images (n>120 cells from 3 independent experiments, ns p > 0.05, ** p < 0.005, data is presented as mean ± SEM). (C) Representative trajectories of FACS sorted MCF7 cells migrating on E-selectin coated cell culture plate in the presence of 0.4 U/ml neuraminidase and 20 µg/ml Tunicamycin (NMase) or vehicle (DMSO). (D) Quantification of 2D speed (n=3, 55-70 cells per condition, ** p < 0.005, data is presented as mean ± SEM). (E) Adhesion assay with MUC1 FACS sorted MCF7 cells on E-selectin coated surface. Cells seeded on coated 96-well plate were allowed to attach for 20 minutes, was then PBS washed and was stained with DAPI and the whole plate was imaged using microscope to count cells. Representative images of nucleus in one well after thresholding with top row showing wells without wash step (seed) and the bottom panel showing attached cells with and without NMase treatment. (F) Quantification of attached cell . (n=3, ** p < 0.005, ns p > 0.05, data is presented as mean ± SEM). (G) FACS sorted MCF7 cells seeded on a selectin coated microfluidics channel were subjected to different flow induced shear stress and were imaged using a live cell imaging setup. Representative phase contrast images showing a section of the channel at different time points with attached cells (H) Quantification of remaining attached cell fraction across different conditions as they are subjected to flow induced shear stress (n≥3 data is presented as mean ± SEM). (I) WGA staining shows cell leave cell leaves glycan patch after detachment. WGA stained MCF7 cells seeded on a selectin coated microfluidics channel subjected flow induced shear was imaged using fluorescent live cell imaging setup. Yellow dotted line shows the cell boundary and orange dotted line shows initial cell adhesion area.

Journal: bioRxiv

Article Title: Bulky glycocalyx drives cancer invasiveness by modulating substrate-specific adhesion

doi: 10.1101/2023.08.03.551677

Figure Lengend Snippet: (A) Representative phase contrast images showing morphology of MUC1 FACS sorted MCF7 cells cultured on E-selectin coated substrate. MCF7 cells are sorted in mucin-1 negative, intermediate (low) and high MUC1 expressing cell populations and were grown for 14 hours in presence of DMSO control or 0.4 U/ml Neuraminidase and 20 µg/ml tunicamycin (NMase). (B) Quantification of cell spread area and cell circularity form obtained images (n>120 cells from 3 independent experiments, ns p > 0.05, ** p < 0.005, data is presented as mean ± SEM). (C) Representative trajectories of FACS sorted MCF7 cells migrating on E-selectin coated cell culture plate in the presence of 0.4 U/ml neuraminidase and 20 µg/ml Tunicamycin (NMase) or vehicle (DMSO). (D) Quantification of 2D speed (n=3, 55-70 cells per condition, ** p < 0.005, data is presented as mean ± SEM). (E) Adhesion assay with MUC1 FACS sorted MCF7 cells on E-selectin coated surface. Cells seeded on coated 96-well plate were allowed to attach for 20 minutes, was then PBS washed and was stained with DAPI and the whole plate was imaged using microscope to count cells. Representative images of nucleus in one well after thresholding with top row showing wells without wash step (seed) and the bottom panel showing attached cells with and without NMase treatment. (F) Quantification of attached cell . (n=3, ** p < 0.005, ns p > 0.05, data is presented as mean ± SEM). (G) FACS sorted MCF7 cells seeded on a selectin coated microfluidics channel were subjected to different flow induced shear stress and were imaged using a live cell imaging setup. Representative phase contrast images showing a section of the channel at different time points with attached cells (H) Quantification of remaining attached cell fraction across different conditions as they are subjected to flow induced shear stress (n≥3 data is presented as mean ± SEM). (I) WGA staining shows cell leave cell leaves glycan patch after detachment. WGA stained MCF7 cells seeded on a selectin coated microfluidics channel subjected flow induced shear was imaged using fluorescent live cell imaging setup. Yellow dotted line shows the cell boundary and orange dotted line shows initial cell adhesion area.

Article Snippet: For probing focal adhesion dynamics, mCherry Paxillin transfected cells were seeded on collagen-coated glass bottom dishes and imaged at 30 sec intervals for 30-45 min at 63× magnification using a scanning probe confocal microscope (Zeiss, LSM 780) .

Techniques: Cell Culture, Expressing, Control, Cell Adhesion Assay, Staining, Microscopy, Shear, Live Cell Imaging, Glycoproteomics

(A) Representative phase contrast images showing morphology of MUC1 FACS sorted MCF7 cells cultured on collagen-coated substrate. MCF7 cells are sorted in mucin-1 negative, intermediate (low) and high MUC1 expressing cell populations and were grown for 14 hours in presence of DMSO control or 0.4 U/ml Neuraminidase and 20 µg/ml tunicamycin (NMase). (B) Quantification of cell spread area and cell circularity form obtained images (n>100 cells from 3 independent experiments, ns p > 0.05, ** p < 0.005, data is presented as mean ± SEM). (C) Schematic of adhesion assay with MUC1 FACS sorted MCF7 cells. Facs sorted MCF7 cells are seeded in collagen coated 96-well plate and were allowed to attach for 20 minutes, was then PBS washed and was stained with DAPI and the whole plate was imaged using microscope to count cells. (D) Representative images of nucleus in one well after thresholding with top row showing wells without wash step (seed) and the bottom panel is showing attached cells with and without NMase treatment. (E) Quantification of attached cell fraction . (n=3, ** p < 0.005, ns p > 0.05, data is presented as mean ± SEM). (F-H) De-adhesion assay of MUC1 FACS sorted MCF7 cells without and with enzymatic deglycosylation using neuraminidase treatment (NMase). (F) Schematics of the de-adhesion assay. (G) Representative frames showing cell de-adherence over time and (H) Graph is showing average de-adhesion time (n=3, 90 - 150 cells per condition, ** p < 0.005, NS p > 0.05, data is presented as mean ± SEM).

Journal: bioRxiv

Article Title: Bulky glycocalyx drives cancer invasiveness by modulating substrate-specific adhesion

doi: 10.1101/2023.08.03.551677

Figure Lengend Snippet: (A) Representative phase contrast images showing morphology of MUC1 FACS sorted MCF7 cells cultured on collagen-coated substrate. MCF7 cells are sorted in mucin-1 negative, intermediate (low) and high MUC1 expressing cell populations and were grown for 14 hours in presence of DMSO control or 0.4 U/ml Neuraminidase and 20 µg/ml tunicamycin (NMase). (B) Quantification of cell spread area and cell circularity form obtained images (n>100 cells from 3 independent experiments, ns p > 0.05, ** p < 0.005, data is presented as mean ± SEM). (C) Schematic of adhesion assay with MUC1 FACS sorted MCF7 cells. Facs sorted MCF7 cells are seeded in collagen coated 96-well plate and were allowed to attach for 20 minutes, was then PBS washed and was stained with DAPI and the whole plate was imaged using microscope to count cells. (D) Representative images of nucleus in one well after thresholding with top row showing wells without wash step (seed) and the bottom panel is showing attached cells with and without NMase treatment. (E) Quantification of attached cell fraction . (n=3, ** p < 0.005, ns p > 0.05, data is presented as mean ± SEM). (F-H) De-adhesion assay of MUC1 FACS sorted MCF7 cells without and with enzymatic deglycosylation using neuraminidase treatment (NMase). (F) Schematics of the de-adhesion assay. (G) Representative frames showing cell de-adherence over time and (H) Graph is showing average de-adhesion time (n=3, 90 - 150 cells per condition, ** p < 0.005, NS p > 0.05, data is presented as mean ± SEM).

Article Snippet: For probing focal adhesion dynamics, mCherry Paxillin transfected cells were seeded on collagen-coated glass bottom dishes and imaged at 30 sec intervals for 30-45 min at 63× magnification using a scanning probe confocal microscope (Zeiss, LSM 780) .

Techniques: Cell Culture, Expressing, Control, Cell Adhesion Assay, Staining, Microscopy